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aml 12 cells  (ATCC)


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    ATCC aml 12 cells
    Aml 12 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1651 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aml+12+cells/AML12/pm42092182-285-0-2
    Average 98 stars, based on 1651 article reviews
    aml 12 cells - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Cell Culture:

    Article Title: NEK7 couples SDHB to orchestrate respiratory chain electron transport homeostasis that impedes liver fibrosis
    Article Snippet: HepaRG (Shanghai Hong Shun Biological Technology, C0986, from Millipore) and LX-2 (Procell, CL-0560) cells were maintained in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco). .. Aml-12 cells (SUNNCELL, SNL-242, from ATCC) were cultured in DMEM-F12 medium (Gibco) supplemented with 10% fetal bovine serum (PAN-biotech). .. Subcultures were performed using 0.25% trypsin–EDTA (Gibco).

    Article Title: Hepatocyte Circadian Clocks Control Cholesterol Metabolism and Protect From Metabolic Dysfunction-Associated Steatohepatitis (MASH)
    Article Snippet: .. AML-12 cells, obtained from ATCC Biobank (catalog no. CRL-2254), were cultured in Gibco Dulbecco’s Modified Eagle Medium supplemented with Nutrient Mixture F12 (DMEM/F12, ThermoFisher), containing 1% penicillin-streptomycin, 1% insulin-transferrin-selenium (ITS, ThermoFisher), 10% non-heat-inactivated fetal bovine serum (FBS, ThermoFisher), and 10 nM dexamethasone (Sigma-Aldrich). ..

    Article Title: HlncRNA-1 integrates the hepatic circadian clock with lipid metabolism by targeting Hdlbp.
    Article Snippet: Cell culture and RNA interference Primary mouse hepatocytes were isolated via collagenase IV (Gibco, USA) perfusion and maintained in highglucose DMEM (Gibco, USA) 10% fetal bovine serum (FBS) at 37°C with 5% CO2. .. AML-12 cells (ATCC, USA) were cultured in DMEM/F12 (Procell, China) supplemented with 10% FBS and ITS-X (Procell, China) under identical incubation conditions (37°C, 5% CO2). .. For individual gene silencing, cells were transfected with siRNAs targeting HlncRNA-1 or Hdlbp (GenePharma, China), alongside respective non-targeting controls.

    Modification:

    Article Title: Hepatocyte Circadian Clocks Control Cholesterol Metabolism and Protect From Metabolic Dysfunction-Associated Steatohepatitis (MASH)
    Article Snippet: .. AML-12 cells, obtained from ATCC Biobank (catalog no. CRL-2254), were cultured in Gibco Dulbecco’s Modified Eagle Medium supplemented with Nutrient Mixture F12 (DMEM/F12, ThermoFisher), containing 1% penicillin-streptomycin, 1% insulin-transferrin-selenium (ITS, ThermoFisher), 10% non-heat-inactivated fetal bovine serum (FBS, ThermoFisher), and 10 nM dexamethasone (Sigma-Aldrich). ..

    Incubation:

    Article Title: HlncRNA-1 integrates the hepatic circadian clock with lipid metabolism by targeting Hdlbp.
    Article Snippet: Cell culture and RNA interference Primary mouse hepatocytes were isolated via collagenase IV (Gibco, USA) perfusion and maintained in highglucose DMEM (Gibco, USA) 10% fetal bovine serum (FBS) at 37°C with 5% CO2. .. AML-12 cells (ATCC, USA) were cultured in DMEM/F12 (Procell, China) supplemented with 10% FBS and ITS-X (Procell, China) under identical incubation conditions (37°C, 5% CO2). .. For individual gene silencing, cells were transfected with siRNAs targeting HlncRNA-1 or Hdlbp (GenePharma, China), alongside respective non-targeting controls.



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    Elevated GATA2 protein expression in hepatocytes under endoplasmic reticulum stress and oxidative stress. A: Cell viability assessed by Cell Counting Kit-8 assay in <t>thapsigargin-treated</t> <t>AML-12</t> cells; B: Semi-quantitative analysis of thapsigargin-induced GATA2 protein expression by western blotting (representative blots shown); C and E: Cell viability measured by Cell Counting Kit-8 assay in AML-12 (C) and HepG2 cells (E) treated with H 2 O 2 ; D and F: Semi-quantitative analysis of H 2 O 2 -induced GATA2 protein expression by western blotting in AML-12 (D) and HepG2 cells (F) (representative blots shown). b P < 0.01 vs control group. HSP60: Heat shock protein 60.
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    Elevated GATA2 protein expression in hepatocytes under endoplasmic reticulum stress and oxidative stress. A: Cell viability assessed by Cell Counting Kit-8 assay in <t>thapsigargin-treated</t> <t>AML-12</t> cells; B: Semi-quantitative analysis of thapsigargin-induced GATA2 protein expression by western blotting (representative blots shown); C and E: Cell viability measured by Cell Counting Kit-8 assay in AML-12 (C) and HepG2 cells (E) treated with H 2 O 2 ; D and F: Semi-quantitative analysis of H 2 O 2 -induced GATA2 protein expression by western blotting in AML-12 (D) and HepG2 cells (F) (representative blots shown). b P < 0.01 vs control group. HSP60: Heat shock protein 60.
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    Image Search Results


    Elevated GATA2 protein expression in hepatocytes under endoplasmic reticulum stress and oxidative stress. A: Cell viability assessed by Cell Counting Kit-8 assay in thapsigargin-treated AML-12 cells; B: Semi-quantitative analysis of thapsigargin-induced GATA2 protein expression by western blotting (representative blots shown); C and E: Cell viability measured by Cell Counting Kit-8 assay in AML-12 (C) and HepG2 cells (E) treated with H 2 O 2 ; D and F: Semi-quantitative analysis of H 2 O 2 -induced GATA2 protein expression by western blotting in AML-12 (D) and HepG2 cells (F) (representative blots shown). b P < 0.01 vs control group. HSP60: Heat shock protein 60.

    Journal: World Journal of Stem Cells

    Article Title: GATA2 deficiency exacerbates chronic liver injury via disrupting hepatocyte death-regeneration balance: Clinical, histopathological, and molecular evidence

    doi: 10.4252/wjsc.v18.i2.112940

    Figure Lengend Snippet: Elevated GATA2 protein expression in hepatocytes under endoplasmic reticulum stress and oxidative stress. A: Cell viability assessed by Cell Counting Kit-8 assay in thapsigargin-treated AML-12 cells; B: Semi-quantitative analysis of thapsigargin-induced GATA2 protein expression by western blotting (representative blots shown); C and E: Cell viability measured by Cell Counting Kit-8 assay in AML-12 (C) and HepG2 cells (E) treated with H 2 O 2 ; D and F: Semi-quantitative analysis of H 2 O 2 -induced GATA2 protein expression by western blotting in AML-12 (D) and HepG2 cells (F) (representative blots shown). b P < 0.01 vs control group. HSP60: Heat shock protein 60.

    Article Snippet: Human hepatocellular carcinoma HepG2 cells and mouse hepatocyte AML-12 cells were purchased from the American Type Culture Collection and authenticated via short tandem repeat profiling.

    Techniques: Expressing, Cell Counting, Western Blot, Control